The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SFRP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SFRP4. Next,...

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

Secreted Frizzled Related Protein 4 (SFRP4) BioAssayT ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro quantitative measurement of SFRP4 in mouse serum, plasma and other biological fluids. Detection Range: 0.156-10ng/ml, Sensitivity: 0.063ng/ml, Intra-Assay CV: <10%, Inter-Assay CV: <12%, Kit...

Mouse sFRP4 Recombinant Protein (RPES4983) is a highly pure recombinant protein developed by Assay Genie for use in a range of applications Protein function: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell...

Protein function: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types (PubMed:27355534). SFRP4 plays a role in bone morphogenesis (PubMed:27355534). May also act...